Nephro-Urology Monthly

Published by: Kowsar

Olive Leaves Extract Improved Sperm Quality and Antioxidant Status in the Testis of Rat Exposed to Rotenone

Maryam Sarbishegi 1 , 2 , Enam Alhagh Charkhat Gorgich 3 , 4 , * and Ozra Khajavi 2
Authors Information
1 Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
2 Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
3 Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
4 Student Research Committee, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
Article information
  • Nephro-Urology Monthly: May 2017, 9 (3); e47127
  • Published Online: April 26, 2017
  • Article Type: Research Article
  • Received: March 1, 2017
  • Accepted: April 10, 2017
  • DOI: 10.5812/numonthly.47127

To Cite: Sarbishegi M, Alhagh Charkhat Gorgich E, Khajavi O. Olive Leaves Extract Improved Sperm Quality and Antioxidant Status in the Testis of Rat Exposed to Rotenone, Nephro-Urol Mon. 2017 ; 9(3):e47127. doi: 10.5812/numonthly.47127.

Copyright © 2017, Nephrology and Urology Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Johren O, Dendorfer A, Dominiak P. Cardiovascular and renal function of angiotensin II type-2 receptors. Cardiovasc Res. 2004; 62(3): 460-7[DOI][PubMed]
  • 2. Omar SH. Oleuropein in olive and its pharmacological effects. Sci Pharm. 2010; 78(2): 133-54[DOI][PubMed]
  • 3. Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev. 1998; 56(11): 317-33[PubMed]
  • 4. Sarbishegi M, Mehraein F, Soleimani M. Antioxidant role of oleuropein on midbrain and dopaminergic neurons of substantia nigra in aged rats. Iran Biomed J. 2014; 18(1): 16-22[PubMed]
  • 5. Lopez-Miranda J, Perez-Jimenez F, Ros E, De Caterina R, Badimon L, Covas MI, et al. Olive oil and health: summary of the II international conference on olive oil and health consensus report, Jaen and Cordoba (Spain) 2008. Nutr Metab Cardiovasc Dis. 2010; 20(4): 284-94[DOI][PubMed]
  • 6. Mehraein F, Sarbishegi M, Aslani A. Therapeutic effects of oleuropein on wounded skin in young male BALB/c mice. Wounds. 2014; 26(3): 83-8[PubMed]
  • 7. Hamdi HK, Castellon R. Oleuropein, a non-toxic olive iridoid, is an anti-tumor agent and cytoskeleton disruptor. Biochem Biophys Res Commun. 2005; 334(3): 769-78[DOI][PubMed]
  • 8. Durlu-Ozkaya F, Ozkaya MT. Oleuropein using as an additive for feed and products used for humans. J Food Process Technol. 2011; 2(3)
  • 9. Visioli F, Bellomo G, Galli C. Free radical-scavenging properties of olive oil polyphenols. Biochem Biophys Res Commun. 1998; 247(1): 60-4[DOI][PubMed]
  • 10. Chimi H, Cillard J, Cillard P, Rahmani M. Peroxyl and hydroxyl radical scavenging activity of some natural phenolic antioxidants. J American Oil Chemists Society. 1991; 68(5): 307-12[DOI]
  • 11. Ferroni F, Maccaglia A, Pietraforte D, Turco L, Minetti M. Phenolic antioxidants and the protection of low density lipoprotein from peroxynitrite-mediated oxidations at physiologic CO2. J Agric Food Chem. 2004; 52(10): 2866-74[DOI][PubMed]
  • 12. Strzezek R, Koziorowska-Gilun M, Stawiszynska M. Cryopreservation of canine semen: the effect of two extender variants on the quality and antioxidant properties of spermatozoa. Pol J Vet Sci. 2012; 15(4): 721-6[PubMed]
  • 13. Zahkok S, Elnaga NA, Ismail AF, Mousa E. Studies on fertility of diabetic male rats treated with olive leaves extract. J Biomed Pharm Res. 2016; 5(3)
  • 14. Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol. 2008; 59(1): 2-11[DOI][PubMed]
  • 15. Maneesh M, Jayalekshmi H. Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction. Indian J Clin Biochem. 2006; 21(2): 80-9[DOI][PubMed]
  • 16. Aitken RJ, Smith TB, Lord T, Kuczera L, Koppers AJ, Naumovski N, et al. On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid. Andrology. 2013; 1(2): 192-205[DOI][PubMed]
  • 17. Al-Haj M, Nasser A, Anis A. Survey of pesticides used in Qat cultivation in Dhale and Yafe and their adverse effects. 2005 9: 103-10
  • 18. Swarnkar S, Singh S, Mathur R, Patro IK, Nath C. A study to correlate rotenone induced biochemical changes and cerebral damage in brain areas with neuromuscular coordination in rats. Toxicology. 2010; 272(1-3): 17-22[DOI][PubMed]
  • 19. Santiago RM, Barbieiro J, Lima MM, Dombrowski PA, Andreatini R, Vital MA. Depressive-like behaviors alterations induced by intranigral MPTP, 6-OHDA, LPS and rotenone models of Parkinson's disease are predominantly associated with serotonin and dopamine. Prog Neuropsychopharmacol Biol Psychiatry. 2010; 34(6): 1104-14[DOI][PubMed]
  • 20. Mohammadi M, Alipour M, Alipour MR, Vatankhah A. Effects of high cholesterol diet and parallel chronic exercise on erythrocyte primary antioxidant enzymes and plasma total antioxidant capacity in Dutch rabbits. Inter J Endocrinology Metabol. 2006; : 30-40
  • 21. Abbasi MM, Dadkhah N, Shahnazi M, Parvin N. Protective effects of melissa officinalis (lemon balm) on sperm parameters and spermatogenesis quality in rats exposed to lead. Iran Red Crescent Med J. 2016; 18(12)
  • 22. Esmaeili-Mahani S, Rezaeezadeh-Roukerd M, Esmaeilpour K, Abbasnejad M, Rasoulian B, Sheibani V, et al. Olive (Olea europaea L.) leaf extract elicits antinociceptive activity, potentiates morphine analgesia and suppresses morphine hyperalgesia in rats. J Ethnopharmacol. 2010; 132(1): 200-5[DOI][PubMed]
  • 23. Asadi MH, Zafari F, Sarveazad A, Abbasi M, Safa M, Koruji M, et al. Saffron improves epididymal sperm parameters in rats exposed to cadmium. Nephrourol Mon. 2014; 6(1)[DOI][PubMed]
  • 24. Mansour SW, Sangi S, Harsha S, Khaleel MA, Ibrahim AR. Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract. Asian Pac J Trop Biomed. 2013; 3(7): 563-8[DOI][PubMed]
  • 25. Bjorndahl L, Soderlund I, Kvist U. Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment. Hum Reprod. 2003; 18(4): 813-6[PubMed]
  • 26. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1): 70-6[DOI][PubMed]
  • 27. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2): 351-8[PubMed]
  • 28. Sanders LH, Greenamyre JT. Oxidative damage to macromolecules in human Parkinson disease and the rotenone model. Free Radic Biol Med. 2013; 62: 111-20[DOI][PubMed]
  • 29. von Wrangel C, Schwabe K, John N, Krauss JK, Alam M. The rotenone-induced rat model of Parkinson's disease: behavioral and electrophysiological findings. Behav Brain Res. 2015; 279: 52-61[DOI][PubMed]
  • 30. Bashkatova V, Alam M, Vanin A, Schmidt WJ. Chronic administration of rotenone increases levels of nitric oxide and lipid peroxidation products in rat brain. Exp Neurol. 2004; 186(2): 235-41[DOI][PubMed]
  • 31. Terzi A, Iraz M, Sahin S, Ilhan A, Idiz N, Fadillioglu E. Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue. Toxicol Ind Health. 2004; 20(6-10): 141-7[DOI][PubMed]
  • 32. Alirezaei M, Kheradmand A, Heydari R, Tanideh N, Neamati S, Rashidipour M. Oleuropein protects against ethanol-induced oxidative stress and modulates sperm quality in the rat testis. Med J Nutrition Metab. 2012; 5(3): 205-11
  • 33. Kumral A, Giris M, Soluk-Tekkesin M, Olgac V, Dogru-Abbasoglu S, Turkoglu U, et al. Effect of olive leaf extract treatment on doxorubicin-induced cardiac, hepatic and renal toxicity in rats. Pathophysiology. 2015; 22(2): 117-23[DOI][PubMed]
  • 34. Nasirzadeh M, Rasouli A. Pretreatment effect of alcoholic olive leaf extract on oxidative and antioxidative enzymes status in ovariectomized rats. Inter J Women's Health Reprod Sci. 2015; 4(2): 77-80[DOI]
  • 35. Sarbishegi M, Heidari Z, Mahmoudzadeh-Sagheb H, Valizadeh M, Doostkami M. Neuroprotective effects of Withania coagulans root extract on CA1 hippocampus following cerebral ischemia in rats. Avicenna J Phytomed. 2016; 6(4): 399-409[PubMed]
  • 36. Khosrowbeygi A, Zarghami N, Deldar Y. Correlation between sperm quality parameters and seminal plasma antioxidants status. Intern J Reproductive BioMed. 2012; 2(2): 58-64
  • 37. Fujii J, Iuchi Y, Matsuki S, Ishii T. Cooperative function of antioxidant and redox systems against oxidative stress in male reproductive tissues. Asian J Androl. 2003; 5(3): 231-42[PubMed]
  • 38. Minguez-Alarcon L, Mendiola J, Lopez-Espin JJ, Sarabia-Cos L, Vivero-Salmeron G, Vioque J, et al. Dietary intake of antioxidant nutrients is associated with semen quality in young university students. Hum Reprod. 2012; 27(9): 2807-14[DOI][PubMed]
  • 39. Gharagozloo P, Aitken RJ. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Hum Reprod. 2011; 26(7): 1628-40[DOI][PubMed]
  • 40. Wang Y, Wang S, Cui W, He J, Wang Z, Yang X. Olive leaf extract inhibits lead poisoning-induced brain injury. Neural Regen Res. 2013; 8(22): 2021-9[DOI][PubMed]
  • 41. Rafighdoost H, Tavafi M, Rasoulian B, Ahmadvand H, Mahmodi M, Rashidi Pour M. Effect of olive leaf extract ininhibition of renal ischemia-reperfusion injuries in rat. Anatomical Sci J. 2013; 10(3): 160-5
  • 42. Tavafi M, Ahmadvand H, Toolabi P. Inhibitory effect of olive leaf extract on gentamicin-induced nephrotoxicity in rats. Iran J Kidney Dis. 2012; 6(1): 25-32[PubMed]
  • 43. Hong CY, Chiang BN, Wei YH. Mitochondrial respiration inhibitors and human sperm motility: implication in the development of spermicides. Br J Clin Pharmacol. 1983; 16(5): 487-90[PubMed]
  • 44. Scola G, Kim HK, Young LT, Andreazza AC. A fresh look at complex I in microarray data: clues to understanding disease-specific mitochondrial alterations in bipolar disorder. Biol Psychiatry. 2013; 73(2)-5[DOI][PubMed]
  • 45. Xiao S, Wan-Xi Y. Mitochondria: transportation, distribution and function during spermiogenesis. Adv Biosci Biotechnol. 2010;
  • 46. Cassina A, Silveira P, Cantu L, Montes JM, Radi R, Sapiro R. Defective human sperm cells are associated with mitochondrial dysfunction and oxidant production 1. Biol reproduct. 2015; 93(5): 1-10
  • 47. Plaza Davila M, Martin Munoz P, Tapia JA, Ortega Ferrusola C, Balao da Silva CC, Pena FJ. Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility. PLoS One. 2015; 10(9)[DOI][PubMed]
  • 48. Radad K, Moldzio R, Rausch WD. Rapamycin protects dopaminergic neurons against rotenone-induced cell death in primary mesencephalic cell culture. Folia Neuropathol. 2015; 53(3): 250-61[DOI][PubMed]
  • 49. Buhlman LM. Parkin loss-of-function pathology: Premature neuronal senescence induced by high levels of reactive oxygen species? Mech Ageing Dev. 2017; 161: 112-20[DOI][PubMed]
  • 50. Sawyer DE, Mercer BG, Wiklendt AM, Aitken RJ. Quantitative analysis of gene-specific DNA damage in human spermatozoa. Mutat Res Fund Mol Mech Mut. 2003; 529(1-2): 21-34[DOI]
  • 51. Najafizadeh P, Dehghani F, Panjeh Shahin M, Hamzei Taj S. The effect of a hydro-alcoholic extract of olive fruit on reproductive argons in male sprague-dawley rat. Inter J Reproduct BioMed. 2013; 11(4): 293-300
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